neuronal cultures. 67 Another effective strategy is the co‐culture of neurons with supporting cells, such as human astrocytes or primary mouse astrocytes. 28 , 39 This co‐culture approach promotes not only maturation, but also synchronization of neuronal activity. 68 , 69 In line with this, co‐cultures of human iPSC‐derived neurons and oligodendrocytes, which allow the study of neuron–glia cross‐talk and myelination processes, led to the observation of neuronal alterations related to tuberous sclerosis complex that were not apparent in neuronal monocultures. 70 These issues show that iPSC‐derived neurons, while invaluable for studying both coding and noncoding gene variants in the context of a human genetic background, should be optimized for the questions being asked and interpreted with care.
