scatter (SS). We defined the cell population with the FS and SS parameters, MitoSOX Red was excited by laser at 488 nm, and its fluorescence emission was collected at 620 nm. In order to perform the analysis in live cells, a gate in the dot plot FS vs. SS was used, where cell debris was gated out for the analysis. We also used the LIVE/DEAD®; Fixable Near-IR Dead Cell Stain Kit (Molecular Probes) to determine the viability of cells, and only the live cell population was used for the analysis. Data were represented as the [mean ± SEM], showing the percentage of gated mean intensity of MitoSOX fluorescence expressed as a percentage when comparing untreated or DMSO vehicle control-cells with MitoSOX positive-cells.
