The analytes were detected through single reaction monitoring (as [M + Ag] + complexes except AA, which is ionized as [(M—H)+2Ag]+) in the positive ion mode using the following reactions (the mass in parentheses represents the mass of the deuterated internal standard): AA (m/z 519(527)→409(417)); 2-AG (m/z 485(493)→411(419)); SAG (m/z 751(759)→411(419)) and OAG (m/z 759→411). Quantification was achieved through stable isotope dilution for AA, 2-AG, and SAG whereas OAG was quantified relative to SAG-d8. Levels of analytes are given in pmols of analyte per mg wet tissue weight.
