Chunk #4 — Methods and Materials — Animals and Tissue Processing — Generation of CB1R heterozygous and knockout mice

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Cortical glutamic acid decarboxylase 67 deficiency results in lower cannabinoid 1 receptor messenger RNA expression: implications for schizophrenia.

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Coronal sections from all mouse brains were cut on a cryostat at 12 μm, thaw mounted onto SupraFrost slides (Fisher Scientific, Pittsburgh, PA), and stored at −80°C until used.