Plasma was collected from fed WT and Tg 10-week old male mice, based on glucose values, mice were separated into 3 groups (N = 8) per genotype such that the average intra-genotype blood glucose levels were similar. Each group was intraperitoneally (IP) injected with 1 or 10 mg/kg body weight/day rmuFGF21 or vehicle bis in die (BID) for 21 days at which point the mice were necropsied. Body composition was determined on treatments days 0, 7, and 14. A second fed plasma sample was collected after 14 days of treatment and GTTs were performed after 18 days of treatment (both 1 hour after dosing). At necropsy (day 21), liver, WAT (epididymal and inguinal) and BAT were excised, weighed and flash frozen for further analysis.
