Chunk #9 — Methods — Anti-sense RNA synthesis, amplification, purification and hybridization to Illumina expression chips

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Complex nature of SNP genotype effects on gene expression in primary human leucocytes.

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Anti-sense RNA was synthesized, amplified and purified using the Ambion Illumina TotalPrep Amplification Kit (Ambion, USA) following the manufacturers' protocol. Complementary RNA was hybridized to Illumina HumanRef-8 v2 Whole Genome BeadChips and scanned on the Illumina BeadArray Reader. Data was handled through the Illumina BeadStudio Gene Expression module v3.2. All gene expression (MIAME compliant) and genotype data has been made freely available by submission to GEO under GSE11501.