Blood and brains were collected at either 6 hours or 3 days after LPS injection. The brains were split into two hemispheres. One hemisphere was quickly frozen and kept at −80°C until further biochemical and mRNA analyses. The other hemisphere was fixed with 4% paraformaldehyde solution. Coronal brain sections (20 μm thick) were made in a cryostat and eight equidistant sections with 0.5 mm intervals mounted on a glass slide for histological analyses.
