By default, g:GOSt uses the set of all annotated protein-coding genes as a background. In some experiments however, only a subset of genes or proteins is measured. For example, the targeted sequencing of only disease specific genes would imply enrichment of that disease association. Statistically, for these cases it is recommended and sometimes necessary to use custom background information when calculating the statistical enrichment significance. The custom background should include a list of genes that were actually measured during the biological experiment, such as all genes in the sequencing panel. This option allows to calculate a more precise evaluation of functional enrichment.
