For analysis of cell morphology, Z-series stacks of confocal images were taken and a single confocal image slice with the largest soma area for individual GFP+ neurons was used for quantification using NIH ImageJ program. For analysis of neuronal positioning, single section confocal images of GFP+ neurons with DAPI staining were used to determine the cell localization within four areas defined in Figure 4D. All quantifications were carried out in a blind fashion to experimental conditions. Statistic significance was determined by ANOVA.
