We used the ethanol sedation assay to address the potential influence of mini-w expression on ethanol sensitivity and rapid tolerance. We first assessed initial ethanol sensitivity in Akap200 and TGFβR transposon insertion genotypes in addition to strains containing several different Gal4 transgenes, all marked with mini-w in the same genetic background. There was no correlation between ST50s from ethanol sedation assays and expression of mini-w in these studies (Figure 4A). These results suggest that mini-w does not influence ethanol sensitivity measured in ethanol sedation experiments, in contrast to our data from eRING assays (Figure 1).
