all parameters, and cannot simulate Roche/454 data. ART [13], an unpublished cross-platform simulator, also uses context-dependent error models and does assign quality scores. However it appears limited to a single genome and does not allow training on user's own data sets. Thus there is a need for a realistic, cross-platform NGS simulator, as multiple sequencing platforms are likely to persist, each with their own strengths and weaknesses [14].
