Selection strictly on BEC at the end of the 4 hours drinking session on Day 2 of the DID test also produced concomitant increases in g/kg consumption across generations (Figure 1B), which are considered a correlated response to selection. Whereas BEC had more than tripled over generations, intake increased from 4.00 g/kg in S0 to 6.43 g/kg in S11, an increase of 60.8%. Figure 1C shows the percentage of animals in each generation for which BEC exceeded 100 mg%. This value is consistent with behavioral intoxication in mice. By S11, 53% of males and 58% of females exceeded this threshold. There has been no apparent change in average body weight across generations, nor has the general health of the colonies appeared to change (data not shown).
