JASPAR is a regularly maintained, open-access database that stores manually curated high-quality DNA binding profiles of TFs as PFMs. For the past two decades, JASPAR has consistently upheld its core principles of (i) providing high-quality TF binding profiles, (ii) fostering open access, and (iii) ensuring ease of use. These core principles drove its evolution and growth and contributed to JASPAR’s usefulness in the scientific community studying gene transcription regulation. JASPAR is now a standard resource in computational regulatory genomics (Supplementary Figure S1).