Exome enrichment was performed on 3 µg of genomic DNA, using the SureSelect Human Exome Kit (Agilent Technologies, Inc., Santa Clara, CA), according to the manufacturer's protocol. The kit covers exonic sequences of ∼18,500 genes and a total of ∼33 Mb of target territory. The captured, purified and amplified library targeting the exome from each patient was sequenced on the Illumina GA II. Paired-end sequences were obtained at a read length of 72 bp.
