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Chunk #11 — Methods — Data Analysis

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Prefrontal-hippocampal coupling by theta rhythm and by 2-5 Hz oscillation in the delta band: The role of the nucleus reuniens of the thalamus.
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Local field potentials of the HC, PFC, and nRE during 10 s RPO stimulation were extracted. The middle six seconds of the stimulus was selected, and filtered between 1 and 70 Hz and sampled at 256 Hz. Signals were subjected to Fast Fourier Transform, and power density spectra were obtained. Frequencies with greatest power (peak frequencies) were identified in the HC and PFC, respectively. In the HC, these frequencies corresponded to theta oscillations; the peak power values were then calculated for the PFC and nRE signals for this previously identified theta oscillation. In the PFC, these frequencies corresponded to the 2–5 Hz oscillations in the delta frequency range; the peak power was then calculated for the HC and nRE signal at this previously identified 2–5 Hz oscillation. Spectra were normalized across experiments whereas the minimum and maximum of the peak power at any of the two frequencies across all stimulation instances for a given electrode in a given experiment was calculated and power values were then corrected by subtracting the minimum and dividing by the range to obtain values between