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Chunk #46 — Methods — Electrophysiological recordings

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Theta and beta synchrony coordinate frontal eye fields and anterior cingulate cortex during sensorimotor mapping.
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We simultaneously recorded the LFP and unit activity in the ACC and FEF while monkeys performed a memory-guided saccade task and a randomly interleaved pro-/anti-saccade task (Fig. 1a–c). FEF and ACC recordings were performed by advancing tungsten microelectrodes (FHC, Bowdoinham, ME) through guide tubes into the brain using software-controlled precision microdrives (NAN Instruments Ltd, Israel) on a daily basis. The microdrives were assembled on a plastic grid with 1 mm interhole distance. At the beginning of each experimental session, 2–4 electrodes were advanced into the FEF and ACC. The coordinates of the ACC recording sites for monkey 1 were 5–17 mm anterior, 8–12 mm dorsal, and 2–4 mm to the right side of the anterior commissure. Similarly, the coordinates of the ACC recording sites for monkey 2 were 7–17 mm anterior, 12–16 mm dorsal, and 2–4 mm to the right side of the anterior commissure. The coordinates of the FEF recording sites for monkey 1 were 1 mm anterior, 8–12 mm dorsal, and 13–16 mm to the right side of the anterior commissure. The coordinates of the FEF recording sites