Baculoviral vector carrying a GFP driven by the Cytomegalovirus (CMV) promoter was obtained from Life Technologies and viral particles were prepared accordingly to manufacture's instruction. For transduction, NSCs were infected with bacculoviral particles at ratio of 500–1,000 Multiplicity of Infection (MOI) for 30 minutes at room temperature on a rocker. Transduced cells were plated on culture dishes without virus removal and cultured over night at 37°C. Viral particles were removed by a medium change next day. Neurons in adherent culture were transduced at the ratio as above and were incubated with the viral particles over night at 37°C. Medium was changed the next day.
