While 64% of all significantly ethanol-responsive genes in the PFC belonged to one of the 61 S-score networks, a Fisher's exact test revealed a subset of networks that were statistically enriched for these genes. These ethanol-responsive gene enriched networks (ErGeNs) are depicted in Figure 3b. Network-based clustering (Figure 3c) and a traditional non-parametric partitioning (Figure S3) of all significantly ethanol responsive genes, both revealed the existence of several modules of co-expressed genes that were largely subcomponents of these paraclique-derived ErGeNs, most predominantly ErGeN1 and ErGeN3. Taken together, these results suggested that, at the time point employed by these studies, the PFC transcriptional response to acute ethanol was primarily mediated through a relatively small number of highly organized gene networks.
