Chunk #41 — Materials and Methods — Resequencing of nAChR Genes, SNP Selection, and Genotyping

Source: A candidate gene approach identifies the CHRNA5-A3-B4 region as a risk factor for age-dependent nicotine addiction.
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combined UT-WI-LHS cohorts, and the call rate on 2,006 individuals was 99.42% after 29 individuals were excluded for missing genotypes. For the five SNPs genotyped by TaqMan and SNPlex methods in 236 individuals, there were three discordant calls, all in a single individual. For the 87 SNPs genotyped by SNPlex and resequencing in the subset of 192 individuals, the concordance rate was 99.7%.