Chunk #22 — 2. Material and methods — 2.2. Neuronal induction (Fig. 1A–C) — 2.2.2. Lentiviral transduction — Day-1: Transduction

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Rapid Ngn2-induction of excitatory neurons from hiPSC-derived neural progenitor cells.

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Spinfection was used to increase transduction efficiency [36]. The 24-well plate was placed in the plate centrifuge and spun for 1 h at 1000g, 25 °C. Cells were then incubated at 37 °C. Viral medium was replaced with fresh NPC medium after 3–4 h of transduction.