after four consecutive days (4D) of alcohol exposure (Con= 0.159143 ± .021 pixels/μm2, n=6; EtOH= 0.107303 ± .006 pixels/μm2, n=6) and a 27.9 ± 9.1% decrease (p<0.05) for 4D+2 group (Con= 0.134994 ± .008 pixels/μm2, n=7; 4D+2= 0.097371 ± 0.012 pixels/μm2, n=8). DCX expression returned to control levels by one week following the last dose of alcohol (Con=0.18438 ± 0.010 pixels/μm2, n=7; EtOH=0.17263 ± 0.13 pixels/μm2, n=8). Thus, DCX labeling of new migrating neuroblasts, or neurogenesis, was reduced by binge alcohol exposure in an adolescent model of an AUD.
