Images were obtained using a Nikon A1R Multiphoton Upright Confocal Microscope using NIS-Elements Software. Nonsaturating laser power and gain were determined for each channel and held constant throughout an experiment. We used 2-μm Z-sections with a ×20 air objective, and 0.5-μm Z-sections with a ×60 oil objective. Max stack images vary in depth shown. Z-series are available upon request. FIJI was used to apply contrast and brightness settings for visualization purposes.
