Recordings from organotypic hippocampal slice cultures: Slices were used between DIV 7 and DIV 10. Somatic outside-out patches from CA1 pyramidal cells were performed at RT and clamped at −70 mV using a Multiclamp 700B amplifier. Signals were low-pass filtered at 2.5 kHz and sampled at 10 kHz with a DigidData 1322. AMPAR currents were evoked by local application of 0.5 mM glutamate for 2 s in the presence of 250 µM trichlormethiazide to block receptor desensitization. Recordings were made within 24 hours after infection, using 2–3 MΩ glass electrodes filled with an internal solution consisting of the following (in mM): 115 CsMeSO3, 20 CsCl, 10 HEPES, 2.5 MgCl2, 4 Na2-ATP, 0.4 Na-GTP, 10 Na-phosphocreatine, 0.6 EGTA, and 0.1 spermine, pH 7.2. External perfusion medium consisted of (in mM): 119 NaCl, 2.5 KCl, 2.5 CaCl2, 1.3 MgSO4, 2.7 MgCl2, 1 NaH2PO4, 26.2 NaHCO3 and 11 glucose, saturated with 95% O2 and 5% CO2. Data were analyzed with Clampfit 10.0 and Prism 5.0.
