Chunk #21 — Experimental Design — Controls

Source

Efficient, footprint-free human iPSC genome editing by consolidation of Cas9/CRISPR and piggyBac technologies.

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A negative control (omission of gRNA) should be included in the workflow. A useful positive control for newcomers to the protocol is to target the TAZ locus using the sequences outlined in Suppl. Table 1. TAZ, located on the X chromosome, is present in a single copy in XY iPSCs.