2-SNP LD block composed of that SNP and another synonymous SNP, rs279858, in exon 5 of the same gene. Genotyping was performed using a Taqman genotyping method as described previously (Ittiwut et al., 2008). All genotyping was performed in duplicate; genotyped plates with fewer than 3 mismatched samples (of 384; i.e., an error rate of <0.78%) were employed in the analysis; discordant genotypes were discarded. PCR amplification was carried out under the following conditions: 95° C for 10 min, followed by 15 s at 92° C, and then 60 s at 60° C for 40 cycles. Signals were analyzed with an ABI Prism 7900HT sequence detector using software version 2.1 from Applied Biosystems (Foster City, CA).
