Rats were tested during the dark phase of the light/dark cycle in a standard squared plexiglass arena (40.6 cm × 40.6 cm; MED Associates Inc., St. Albans, VT), equipped with the Versamax activity monitor system (AccuScan Instruments, Inc., Colombus, OH). Locomotor activity was recorded for 60 minutes and the number of beam breaks were analyzed in 5 min time intervals. Test subjects were derived from the same breeding as the animals used for qRT-PCR experiments, each test group (N=10-12/treatment/sex) included animals from 5-7 birth litters and no litter effects were detected in the statistical analyses.
