Blood samples were obtained for genetic analysis. The ADH1B rs1229984 variant was genotyped with Sequenom MassArray technology (Sequenom, San Diego, CA, USA) following standard procedures. Several quality control measures were employed. Genetic variants had a genotyping rate of greater than 99% and were in Hardy-Weinberg equilibrium in both the European and African American groups. The program PEDCHECK (O'Connell and Weeks, 1998) was used to examine Mendelian inheritance, and only individuals with no Mendelian inconsistencies were included in the rs1229984 genotyped sample (N=2580, Figure 1).
