Chunk #45 — Online Methods — Variant detection

Source

Sensitive detection of somatic point mutations in impure and heterogeneous cancer samples.

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yes

Text

We find the maximum LODT across all three values of m and in order to set the unknown allelic fraction parameter f, we could use maximum likelihood estimation, i.e. find f that maximizes LODT. However, for computational efficiency, we instead estimate estimatef^MLasf^=#ofmutantreads#oftotalreads.