We developed predictive models similar to those used to model transcriptional activity to explore the relationship between levels of histone modifications and inclusion of exons in alternately spliced transcripts. Even accounting for expression level, H3K36me3 has a positive contribution to exon inclusion, while H3K79me2 has a negative contribution61. By monitoring the RNA populations in the subcellular fractions of K562 cells, we found that essentially all splicing is co-transcriptional 62, further supporting a link between chromatin structure and splicing.
