We queried the 3309 significant cis-eQTL SNPs in the combined GWAS dataset including ECLIPSE, Norway, and NETT-NAS subjects [11]. Using a strict Bonferroni correction, there were two cis-eQTL SNPs significantly associated with COPD at p<0.05/3309 = 1.5e-5 (Table 2). These two SNPs on chromosome 15q25 are located in CHRNA5 and IREB2, genes with known COPD associations.[12], [36] At a nominal threshold of p<0.01, there were 64 cis-eQTL SNPs associated with COPD (Table S2). There were 56 eQTL SNPs associated with COPD at p<0.01 in the meta-analysis of the ECLIPSE, Norway, NETT-NAS GWAS studies (Table S3), as opposed to the combined analysis of individual-level genotype data. Merging the 64 SNPs from the combined GWAS analysis and the 56 SNPs from the GWAS meta-analysis left 76 unique SNPs, which were brought to replication analysis.
