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Chunk #35 — Methods — Spatiotemporal expression of fetal eGenes in human brain

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Expression quantitative trait loci in the developing human brain and their enrichment in neuropsychiatric disorders.
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We used RNA sequencing data from dissected regions of the human pre- and post-natal brain, available through the BrainSpan project (http://www.brainspan.org/), to explore the regional and developmental expression of the fetal eGenes we identified. FPKM values were log transformed (log2[FPKM+ 0.0001]), clustered using Euclidian distances, and heatmaps were made using ggplot2 [66].