RPGM were calculated for the three different GABAB1 gene lengths. After log2 transformation of the RPGM values, we used two-tailed unpaired t-tests. We also calculated genome wide RPGMs for all genes, transcripts, exons, and introns based on RefSeq Genes model. The RPGM value differences between alcoholic and control groups were tested using DEGseq (21). GABAB1 specific data were then selected from the analysis.
