using the miRNeasy Mini Kit (QIAGEN) and quantified with the RNA 6000 Nano Kit (Agilent) on the Agilent Bioanalyzer system. 1 μg of RNA was used for cDNA synthesis using the High Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific). Quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) for pluripotency gene expression was performed using TaqMan reagents and SeV-specific probe-primer sets (Mr04269880_mr; Thermo Fisher Scientific). A subset of iPSC samples were cultured in T25 tissue culture flasks on MEF feeder cells and sent to Cell Line Genetics for cytogenetic analysis with G-banded karyotyping. An average of 20 cells per cell line were analyzed for chromosome integrity.
