Since studies of stem cell derived neuronal cultures can be impeded by ongoing progenitor proliferation and neurogenesis creating a culture with heterogeneous neuronal identities, a Notch signaling inhibitor (N-[N-(3,5-Difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester, DAPT) was used to synchronize the cultures by stimulating differentiation and inhibiting proliferation of all NPCs at the same time3536.
