high- and low-LG mothers. NaBis treatment of DNA samples converts nonmethylated cytosines to uracils, which are then detected as thymidine on subsequent sequencing gels.115 Methylated cytosines are unaffected by NaBis and the differences in methylation status are thus apparent and easily quantifiable on sequencing gels. We found significantly greater methylation of the exon 17 GR promoter sequence in the offspring of the low-LG mothers. These findings are consistent with the hypothesis that maternal effects alter DNA methylation patterns in the offspring.
