Chunk #77 — Method Details — Detection
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Hybridization of amplicon to complimentary barcodes was detected using a Luminex FlexMap 3D flow cytometer, which detects both bead color (i.e., transcript identity) and the biotin label on the probe (i.e. transcript abundance; as measured by the phycoerythrin channel). Analysis was done using a sample volume of 40 μl.