RNA sequencing data of over 3 million high-quality nuclei from around 100 dissections across adult human brain.18 We analyzed this dataset at the cell-type level including 31 superclusters and 461 cell clusters. For all expression datasets, we calculated a metric of gene expression specificity as previously described16; it measures, for each gene, its expression in a specific tissue or cell-type relative to its total expression across all tissues or cell types. As in previous studies,16,74 we utilized the genes with the top 10% specificity values in each tissue or cell-type for the enrichment analyses.
