Intracerebroventricular cannulae were stereotaxically implanted under ketamine/xylazine anesthesia in the lateral ventricle (coordinates 1.2 mm lateral to sagittal suture and 1.0 mm posterior to bregma), as described previously (8,18,26), and demonstrated to be correctly located by methylene blue staining. Rats received either a single administration of the AMPK activator AICAR (Sigma-Aldrich; 5 μg, dissolved in 5 μL of saline) or vehicle (5 μL saline) (8) at 19:00.
