for 1 hour at 150,000 × g and the pellets were resuspended in 10 μl of PBS (RIPA-insoluble/sarkosyl-insoluble fraction). Both RIPA-soluble/sarkosyl-insoluble and RIPA-insoluble/sarkosyl-insoluble fractions were used for immunoelectron microscopy. The same protocol was used to enrich sarkosyl-insoluble p-tau aggregates in AD brain samples.
