Patients with juvenile-onset schizophrenia, as well as healthy young adult controls free of known mental illness, were recruited and skin biopsies obtained from each. Patient identifiers were not available to investigators besides the treating psychiatrist, although age, gender, race, diagnosis and medication history accompanied cell line identifiers. Fibroblasts were isolated from each sample; from these, 11 new independent hiPSC lines were derived from 8 patient samples (5 juvenile-onset schizophrenia patients and 3 healthy gender-matched and age-analogous controls (Table S1). iPSCs were generated using excisable floxed polycistronic hSTEMCCA lentivirus (Somers et al., 2010; Zou et al., 2012) encoding Oct4, Sox2, Klf4 and c-Myc (Takahashi et al., 2007; Welstead et al., 2008). All lines were initially characterized and validated as pluripotent using global transcriptome profiling by RNA sequencing to assess pluripotent gene expression, as well as immunostaining for Oct4, Nanog, and SSEA4. The identity of each iPSC line was confirmed to match the parental donor fibroblasts using short tandem repeat (STR)-based DNA fingerprinting. iPSC line isolates were also karyotyped concurrently with these experiments to confirm genomic integrity. An additional well-characterized hiPSC control
