ranging from 50 to 2,850 cells. For each downsampling, we called peaks using MACS2 and assessed the fraction of peaks identified using the 2,850-cell population that were able to be recovered using each downsampled population (Supplementary Fig. 1d.e). When sampling 950 cells, 75% of peaks detected when using the larger cell population were able to be recovered, while only 18% of peaks were able to be recovered using the 50-cell population, highlighting the need for sufficient sampling of rare cell populations in single-cell chromatin studies.
