study, URB597 (provided by Merck, Inc., & Co., Inc., Rahway, NJ) was first dissolved in DMSO under gentle heat at a concentration equivalent to 250 mg/ml and then diluted to test concentrations with a vehicle composed of 37% polyethylene glycol 200 (Sigma-Aldrich, St. Louis, MO), 42% water hydroxypropyl-β-cyclodextrin 40% solution (Sigma-Aldrich), and 21% sterile water. JZL184 was prepared in a vehicle of 40% polyethylene glycol, 10% Tween-80, and 50% sterile water. Routes and times of administration for each procedure are provided in the specific procedure section.
