Chunk #20 — Methods — Reagents and antibodies

Source

Establishment of human iPSC-based models for the study and targeting of glioma initiating cells.

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The following antibodies were used at the specified concentrations for flow cytometry analysis: mouse anti-human TRA-1–60-FITC 1:10 (560380, BD), mouse anti-human TRA-1–81-APC 1:10 (560793, BD), mouse anti-human CD184 (CXCR4)-APC 1:10 (555976, BD), mouse anti-human CD15-PE and CD15-FITC (561715 and 555401, BD), mouse anti-human CD24-PE 1:10 (560991, BD), mouse anti-human SSEA4-AlexaFluor647 1:10 (560219, BD), mouse anti-human CD44-PE 1:10 (550989, BD), mouse anti-human CD133/2 (293C3)-PE 1:10 (130-090-853, Miltenyi), mouse APC isotype control 1:10 (555751, BD), mouse FITC isotype control 1:10 (555748, BD), mouse PE isotype control 1:10 (555749, BD).