Final plating was done between DIV 28 and 31 of differentiation on poly-L-ornithine (PLO) plus laminin (10 μg/ml, both Sigma) coated multiwell plates or coverslips with or without primary human astrocytes (1:4 ratio astrocytes to NPCs). Since different batches of laminin from two suppliers (Biolamina and Sigma) seemed to cause a lot of variation in the attachment and balance between proliferation and differentiation of cells, we only used laminin from Sigma (three different batches) for all experiments described in this study.
