discontinuity (Figure 3B, p > 0.05, Regression discontinuity test). The p-value distributions demonstrated the degree of discontinuity between subtype pairs (Figure 3C). For every pair, except for D1S and D2S – the subtypes for which we had the lowest sample sizes – the p-value distributions indicated more statistically significant discontinuities than predicted by chance (Figure 3C, p < 0.00001, Benjamini-Hochberg corrected, Binomial test, Methods). These results demonstrate that each identified MSN subtype is characterized by discrete gene expression patterns.
