We performed Pearson’s correlation analysis of ZDHHC5 in LUAD. There were 179 positive associations and 143 negative associations (FDR < 0.01; R > 0.3 for positive, R < −0.3 for negative). Heatmaps (Figure 3A) revealed the top 50 positively and negatively associated genes, respectively. ZDHHC5 maintains GSC self-renewal capacity, promotes GSC tumorigenicity, and is essential for preserving the migratory, invasive, and angiogenic potentials of GSCs (Chen et al., 2017). However, the stemness of ZDHHC5 in LUAD has not been reported yet. Previously, we found a set of 242 LUAD CSC genes based on the mRNAsi index (Zhang et al., 2020). We mapped the CSC genes with ZDHHC5 association results, and INCENP was the only intersecting gene (Figure 3B). The INCENP gene expression was up-regulated in LUAD cases and different among LUAD subtypes; the overall survival analysis results indicated an unfavorable prognosis of INCENP in LUAD (Supplementary Figure 2). It has been reported that ZDHHC5 depletion suppresses cell proliferation and colony formation of some NSCLC cell lines but not of human bronchial epithelial cell (HBEC3) (Tian et al., 2015). We analyzed
