To gain more insight into target genes either directly or indirectly affected by miR-34a overexpression over a temporally defined time course of human neurodevelopment, the miR-Ctrl and miR-34a overexpression lines were differentiated for 8 weeks with total RNA samples collected at t=0, 2, 4, 8 weeks in duplicate and subject to NanoString assay with a probe set consisting of a total of 131 genes, and the data merged with that from the t=6 weeks sets that were done in triplicates (Tables S8–S9). Using a cut off of a change greater than +/− 1.5-fold with miR-34a overexpression compared to the matched miR-Ctrl control, along with the requirement that at least two of the replicates showed concordant results for the particular time period, a total of 70 transcripts (53%) were identified at one more time point over the neurodevelopmental time course as showing differential expression (Table S10). Of these 70 transcripts, 14 were within one of the BD-associated GWAS loci, including both ANK3 and CACNB3, which replicates with digital mRNA counting assay the qRT-PCR results and Western blot results shown in Figure
