M. Schörnig established the NGN2 iPSC lines. M. Schörnig and S.E. generated iN cultures with assistance from A.W. S.E. and W.H. established the selective single-cell dissociation. S.E. generated the time course and d35 scRNA-seq NGN2-iN scRNA-seq data with support from M. Schörnig, M. Santel, and W.H. M. Schörnig, H.-C.L., and M.T.N., with support from S.E. and A.W., generated IHC data. N.N.K. provided guidance to establish the NGN2 iPSC lines. E.T. provided guidance for IHC and iN culture. H.C.L. generated the NGN2 induction time course scRNA-seq data with support from S.E. and M. Santel. Z.H., S.E., and H.C.L. analyzed the scRNA-seq data. H.C.L., Z.H., S.E., B.T., and J.G.C. designed the study and wrote the manuscript.