Gene expression microarray experiments were conducted with biological replication in all samples. Sample processing order was randomized. For each sample, total RNA was extracted at least twice independently, from tissue homogenized in TRIzol reagent, followed by Qiagen RNAeasy cleanup (Qiagen). RNA quality was assessed by Bioanalyzer (minimum RIN = 7). cRNA was produced using the Agilent Low-Input Linear amplification and labeling kit.
