We generated hiPSCs from schizophrenia patients with the 22q11.2 microdeletion (four lines from two patients), using the fibroblasts according to standard methods20, 21, 23 (Supplementary Figure 1a). CGH array analyses revealed that both patients carried a 2.6 Mb-hemizygous deletion at chromosome 22q11.2.21 This should correspond to a typical 3 Mb deletion because the CGH array lacks the probes for detecting regions near the ends of 3 Mb region.26 No other psychiatric disorder-relevant copy number variants1 were found in the two patients (Supplementary Tables 2 and 3). All of the hiPSC lines could be differentiated into neurons through a stage of neurosphere formation (Supplementary Figure 1b). In our protocol, all the cells in neurospheres expressed the neural marker Nestin, suggesting that our neurospheres consisted almost entirely of neural stem or progenitor cells.20, 21, 27 Thus, all the hiPSC lines were suitable for neuronal analyses.
